Case Study

Repeatable 8-channel gravimetric dispense performance.

Gravimetric testing showed low within-channel variation and tight channel-to-channel agreement across all 8 channels.

Summary

The measured data shows strong repeatability and close channel-to-channel agreement, with a small consistent offset suitable for calibration refinement.

Key results

Channels tested

8

Dispenses per channel

10

Between-channel CV

0.18%

Pooled within-channel CV

0.16%

Overall CV

0.22%

Mean channel spread

0.52 µL

Mean dispense by channel with SD error bars

Bar = channel mean, whisker = +/-1 SD, dashed line = 100 µL target

98.598.999.399.7100.1100.5100 µL targetC1C2C3C4C5C6C7C8ChannelDispense volume (µL)

To evaluate multi-channel dispense consistency, we carried out a gravimetric test across all 8 channels at a nominal 100 µL target per dispense.

Each channel was tested repeatedly under the same conditions, generating 10 measurements per channel. The results showed strong repeatability within each channel and close agreement between channels overall.

Across the dataset, pooled within-channel CV was approximately 0.16%, while the CV of channel means was approximately 0.18%. The overall combined CV across all readings was approximately 0.22%. Channel averages fell within a narrow band, with around 0.52 µL separating the highest and lowest mean channel outputs.

All channels showed a small negative bias relative to the 100 µL target, indicating a consistent systematic offset rather than unstable dispensing behaviour. In practice, this suggests the platform is behaving consistently across channels, and channel matching can be improved further through calibration rather than requiring fundamental mechanical correction.

These results demonstrate repeatable multi-channel performance for liquid handling workflows and provide a strong basis for continued calibration and verification across additional volumes, liquids, and consumables.

Per-channel summary

ChannelMeanBiasCV (%)
Channel 199.910-0.0900.230
Channel 299.729-0.2710.171
Channel 399.761-0.2390.116
Channel 499.481-0.5190.119
Channel 599.642-0.3580.162
Channel 699.385-0.6150.141
Channel 799.470-0.5300.107
Channel 899.528-0.4720.173

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